Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping Christina Laufer, Bernd Fischer, Maximilian Billmann, Wolfgang Huber, and Michael Boutros. Nature Methods (2013) 10(5):427-31 doi: 10.1038/nmeth.2436
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چکیده
5 Convert data from plate order to multi-dimensional SGI-arrary 11 5.1 Preliminaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.2 Parse plate barcodes to annotate the plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.3 Reorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
منابع مشابه
Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping Christina Laufer, Bernd Fischer, Maximilian Billman, Wolfgang Huber, and Michael Boutros. Nature Methods (2013) 10(5):427-31 doi: 10.1038/nmeth.2436
5 Convert data from plate order to multi-dimensional SGI-arrary 11 5.1 Preliminaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.2 Parse plate barcodes to annotate the plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5.3 Reorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....
متن کاملExperimental data and documented source code for the paper A Map of Directional Genetic Interactions in a Metazoan Cell Bernd Fischer, Thomas Sandmann, Thomas Horn, Maximilian Billmann, Varun Chaudhary, Wolfgang Huber, and Michael Boutros eLife, in press, http://www.elifesciences.org
6 Quality control 10 6.1 Quality control of features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6.2 Quality control of dsRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 6.3 Discussion of feature and target gene reagent quality control . . . . . . . . . . . . . . . . . . . . . . 13 6.4 Comparison to Rohn et al. . . ...
متن کاملA map of directional genetic interactions in a metazoan cell
Gene-gene interactions shape complex phenotypes and modify the effects of mutations during development and disease. The effects of statistical gene-gene interactions on phenotypes have been used to assign genes to functional modules. However, directional, epistatic interactions, which reflect regulatory relationships between genes, have been challenging to map at large-scale. Here, we used comb...
متن کاملA genetic interaction map of cell cycle regulators
Cell-based RNA interference (RNAi) is a powerful approach to screen for modulators of many cellular processes. However, resulting candidate gene lists from cell-based assays comprise diverse effectors, both direct and indirect, and further dissecting their functions can be challenging. Here we screened a genome-wide RNAi library for modulators of mitosis and cytokinesis inDrosophilaS2 cells. Th...
متن کاملSource data and code for the paper A Map of Directional Genetic Interactions in a Metazoan Cell Bernd Fischer, Thomas Sandmann, Thomas Horn, Maximilian Billman, Varun Chaudhary, Wolfgang Huber, and Michael Boutros eLife, in press, http://www.elifesciences.org
6 Quality control 10 6.1 Quality control of features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6.2 Quality control of dsRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 6.3 Discussion of feature and target gene reagent quality control . . . . . . . . . . . . . . . . . . . . . . 13 6.4 Comparison to Rohn et al. . . ...
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تاریخ انتشار 2016